Isolation of a plasmid responsible for caseinase activity in Clostridium perfringens ATCC 3626B
نویسندگان
چکیده
منابع مشابه
Construction of an Escherichia coli-Clostridium perfringens shuttle vector and plasmid transformation of Clostridium perfringens.
A stable shuttle vector which replicates in Escherichia coli and Clostridium perfringens was constructed by ligating a 3.6-kilobase (kb) fragment of plasmid pBR322 with C. perfringens plasmid pHB101 (3.1 kb). The marker for this shuttle plasmid originated from the 1.3-kb chloramphenicol resistance gene of plasmid pHR106. The resulting shuttle vector, designated pAK201, is 8 kb in size and codes...
متن کاملLocalization of lecithinase activity in Clostridium perfringens.
The lecithinase of Clostridium perfringens is an exoenzyme (3). However, lecithinase activity was demonstrated in cytoplasmic extracts obtained from mechanically disrupted vegetative cells of type A C. perfringens (4). This report deals with the intracellular and extracellular lecithinase activities of vegetative cells and spores of type A C. perfringens. Strain BP6K (1), which produces conside...
متن کاملIsolation and function of a Clostridium perfringens enterotoxin fragment.
A fragment was obtained by treating Clostridium perfringens enterotoxin with 2-nitro-5-thiocyanobenzoic acid, a reagent which specifically cleaves the amino-terminal peptide bond of cysteine residues. The fragment (molecular weight, 15,000) was purified by high-performance liquid chromatography. The fragment had no cytotoxic effect on Vero cells but competitively inhibited enterotoxin-induced 5...
متن کاملIsolation, identification, and antimicrobial susceptibility of Clostridium perfringens isolates from acute necrotic enteritis of broiler chickens
The aim of this study was to isolate, identify and determine the antimicrobial susceptibility of Clostridium perfringens (CP) isolates from acute necrotic enteritis of broiler chickens. All broiler carcasses diagnosed as necrotic enteritis (NE) were sampled, subjected to microbial tests and 40 isolates were identified according to standard procedures. The antimicrobial susceptibility of CP isol...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1981
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.147.1.262-266.1981